The RRB Lab Technician (Lab Assistant Grade II) Question Paper 2026 with the official answer key is now available. Railway Recruitment Boards conducted the CBT for Paramedical Categories, CEN No. 03/2025 from 11 to 13 March 2026. This post covers the 11 March 2026 (4:30 PM – 6:00 PM) paper of Lab Assistant Grade II — with the question paper PDF, section-wise and topic-wise analysis, 30 important questions with answers, exam pattern, syllabus and cut off details for DMLT / Lab Technician students.
Quick Answer: The RRB Lab Assistant Grade II paper of 11 March 2026 had 100 questions of 100 marks in 90 minutes — 30 General Ability questions (General Awareness 10, Maths & Reasoning 11, General Science 9) and 70 Professional Ability questions. There is 1/3 negative marking. Microbiology (13), Clinical Biochemistry (12) and Blood Banking (10) were the top professional topics. CEN 03/2025 had only 12 Lab Assistant Grade II posts. In the previous recruitment (CEN 04/2024), the official UR cut off for this post was 63.27 to 69.05 normalised marks.
RRB Lab Assistant Grade II Exam 2026 – Key Highlights
Exam Date
11 Mar 2026
Total Questions
100
Lab Assistant Posts
12
Negative Marking
1/3 Mark
RRB Lab Assistant Grade II Exam 2026 – Quick Overview
| Recruiting Body | Railway Recruitment Boards (RRBs), Ministry of Railways |
| Notification | CEN No. 03/2025 – Paramedical Categories (434 posts) |
| Post Name | Lab Assistant Grade II (Lab Technician) |
| Vacancies | 12 posts |
| Pay Level | Level 3 – Initial pay ₹21,700 |
| CBT Dates | 11, 12 and 13 March 2026 |
| Paper Analysed | 11 March 2026, 4:30 PM – 6:00 PM |
| Questions / Marks | 100 questions / 100 marks |
| Duration | 90 minutes (120 minutes for PwBD with scribe) |
| Negative Marking | 1/3 mark for each wrong answer |
| Answer Key | Released on 21 March 2026 (objections till 29 March 2026) |
| CBT Result | Declared on 29 May 2026 |
| Selection Process | CBT → Document Verification → Medical Examination |
RRB Lab Assistant Grade II Question Paper 2026 PDF Download
Download the complete RRB Lab Assistant Grade II question paper of the 11 March 2026 shift below. The PDF has all 100 questions with the correct answers marked as per the official RRB answer key. Solve it in 90 minutes and calculate your score with 1/3 negative marking.
RRB Lab Assistant Grade II Question Paper 2026 PDF
CEN 03/2025 – CBT held on 11 March 2026 (4:30 PM – 6:00 PM) – 100 Questions with Answer Key
⬇ Download Question Paper PDFFree PDF for practice by Testpaperlive Classes. Correct answers as per RRB official answer key.
RRB Lab Assistant Grade II Paper Analysis 2026 (11 March, Shift 3)
After checking all 100 questions, the Testpaperlive faculty team rates the paper as easy to moderate. The General Ability part was mostly bilingual and direct, with current affairs from 2025. The Professional Ability part was practical and lab-oriented — staining, specimen handling, blood bag types, sterilization and biochemistry methods — with a few case-based questions. Because of 1/3 negative marking, avoid guessing.
| Section | Questions | Difficulty Level | Good Attempts |
|---|---|---|---|
| General Awareness | 10 | Moderate | 6–7 |
| Mathematics & Reasoning | 11 | Easy to Moderate | 8–9 |
| General Science | 9 | Easy | 7–8 |
| Professional Ability (Lab Technology) | 70 | Easy to Moderate | 52–57 |
| Total | 100 | Easy to Moderate | 73–81 |
🦠 Microbiology on top
13 questions on Gram stain, ZN, Neisser, India ink, culture media and specimen processing.
🧪 Biochemistry scoring
12 questions on GOD-POD, colorimeter, creatinine, ALT, buffers, molarity and pH.
🩸 Blood bank is a must
10 questions on blood bags, platelet concentrate, donor deferral, anti-D and storage.
🧼 Sterilization & safety
13 questions on autoclave, disinfection, in-use test, lab safety and ethics.
RRB Lab Assistant Grade II Topic-wise Weightage 2026
1. Professional Ability – 70 Questions
Microbiology – 13 Qs
Clinical Biochemistry & Lab Solutions – 12 Qs
Blood Banking & Transfusion – 10 Qs
Sterilization & Disinfection – 7 Qs
Human Anatomy & Physiology – 6 Qs
Lab Safety, Ethics & Management – 6 Qs
Lab Equipment & Glassware – 4 Qs
Specimen Collection & Phlebotomy – 4 Qs
Clinical Pathology (Urine & Stool) – 4 Qs
Haematology – 2 Qs
Histopathology & Molecular – 2 Qs
| Topic | Questions | Concepts Asked |
|---|---|---|
| Microbiology | 13 | Gram stain (iodine step), basic fuchsin counterstain, Ziehl-Neelsen, Neisser stain & metachromatic granules, negative staining with India ink, staphylococci, blood agar for throat swab, CSF for meningitis, urine culture timing, pus smear, sputum rejection, sterility testing of media |
| Clinical Biochemistry & Lab Solutions | 12 | 0.9% saline preparation, molarity, phosphate buffer, pH, GOD-POD method, sodium fluoride & plasma glucose, colorimeter blank & Beer-Lambert law, creatinine, pre-renal azotemia, ALT |
| Blood Banking & Transfusion | 10 | Triple & quadruple bags, platelet concentrate, blood substitutes, bacterial contamination, venipuncture angle, HBV donor deferral, anti-D immunoglobulin, ABO system, whole blood storage (35 days) |
| Sterilization & Disinfection | 7 | Autoclave principle & perforated rack, disinfection definition, in-use test, glutaraldehyde precautions, residue after autoclaving |
| Human Anatomy & Physiology | 6 | Tidal volume & alveolar ventilation, nasal bones, myelin sheath, heart, anatomical terms (medial/lateral), thoracic cavity |
| Lab Safety, Ethics & Management | 6 | Patient confidentiality, malpractice, chemical burns, chemical spill steps, first aid for cuts, lab safety rules |
| Lab Equipment & Glassware | 4 | Serological pipette, laminar airflow cabinet, volumetric flask, pipette calibration with mercury |
| Specimen Collection & Phlebotomy | 4 | Serum vs plasma tubes, haemolysis, lancet, median cubital vein |
| Clinical Pathology | 4 | Urine preservative (formalin), bilirubin in urine, steatorrhoea (2 questions) |
| Haematology | 2 | Microcytic hypochromic anaemia, anaemia due to chronic blood loss |
| Histopathology & Molecular | 2 | Frozen section thickness & albumin adhesive, −80°C storage for DNA |
2. General Ability – 30 Questions
| Section | Questions | Topics Asked |
|---|---|---|
| General Awareness | 10 | Chauri Chaura incident, Article 213, Uniform Civil Code (Part IV), McMahon Line, Mizoram borders, Marathi language family, First Five Year Plan, Online Gaming Act 2025, four Labour Codes, AIIMS Raipur robotic surgery |
| Mathematics | 6 | Quadrilateral area, average speed, recurring decimals, average & ratio, pipes & cistern, successive percentage |
| Reasoning | 5 | Alphabet series, ranking in a row, symbol series, number set, data sufficiency |
| General Science | 9 | Connective tissue, parallel circuits, Fleming's left-hand rule, tissue culture, electron, sound time period, fertilisation, Thomson model, Na₂SO₄ formula |
Important Questions Asked in RRB Lab Assistant Grade II Exam 2026 (with Answers)
Selected questions from the 11 March 2026 paper with answers as per the official RRB answer key.
RRB Lab Assistant Grade II Official Cut Off
RRBs declared the CBT result of CEN 03/2025 on 29 May 2026. The official cut off marks (out of 100, normalised) of candidates shortlisted for Document Verification are published separately by each RRB on its own website. As Lab Assistant Grade II had only 12 posts in CEN 03/2025, its cut off is published only by the RRBs that had vacancies for this post.
Official Cut Off of Previous Recruitment – CEN 04/2024 (Result: 11–12 August 2025)
For comparison, here is the official cut off of Laboratory Assistant Grade II (Category No. 7) published by RRBs in the previous Paramedical recruitment, CEN 04/2024.
| RRB | Railway Unit | UR | SC | ST | OBC | EWS | ESM |
|---|---|---|---|---|---|---|---|
| RRB Ajmer | NWR (North Western Railway) | 63.26531 | 59.52381 | – | 63.26531 | 59.52381 | 48.29932 |
| RRB Mumbai | CR (Central Railway) | 69.04762 | – | – | 67.68707 | – | – |
| RRB Mumbai | WR (Western Railway) | 67.34694 | – | 57.82313 | – | – | – |
| RRB Guwahati | NFR (Northeast Frontier Railway) | – | 58.50340 | – | – | 54.76191 | – |
Note: "–" means no cut off was published in that category for that railway unit. For the exact CEN 03/2025 (2026) Lab Assistant Grade II cut off, check the result & cut off PDF of your RRB on its official website. Minimum qualifying marks as per RRB rules: UR & EWS 40%, OBC & SC 30%, ST 25%.
RRB Lab Assistant Grade II Exam Pattern
| Subject | Questions | Marks |
|---|---|---|
| Professional Ability | 70 | 70 |
| General Awareness | 10 | 10 |
| General Arithmetic, General Intelligence & Reasoning | 10 | 10 |
| General Science | 10 | 10 |
| Total | 100 | 100 |
- Duration: 90 minutes (120 minutes for eligible PwBD candidates with scribe)
- Negative Marking: 1/3 mark for every wrong answer; no deduction for unanswered questions
- Mode: Single-stage Computer Based Test, available in English, Hindi and regional languages
- Normalisation: Marks are normalised because the CBT is held in multiple shifts
RRB Lab Assistant Grade II Syllabus
Professional Ability (70 Questions)
- Human Anatomy & Physiology: body systems, bones, cavities, anatomical terms
- Clinical Biochemistry: glucose, urea, creatinine, LFT, lipid profile, enzymes, colorimetry, buffers, pH, solutions & molarity
- Microbiology: sterilization & disinfection, staining techniques, culture media, specimen processing, bacteria identification
- Haematology & Blood Banking: anaemia, anticoagulants, blood grouping, blood bags & components, donor selection, storage
- Clinical Pathology: urine and stool examination
- Histopathology: fixation, tissue processing, microtomy, frozen sections
- Lab Management: glassware, equipment, lab safety, ethics, first aid, specimen collection
General Ability (30 Questions)
- General Awareness: history, polity, geography, economy, current affairs
- Arithmetic & Reasoning: percentage, average, speed, pipes, series, ranking, coding, data sufficiency
- General Science: Physics, Chemistry and Biology up to Class 10 level
Prepare for RRB Lab Assistant & Lab Technician Exams with Testpaperlive Classes
RRB CEN 05/2026 is coming with new Lab Assistant Grade II vacancies. Join our Lab Technician (DMLT / MLT) online batches — daily live classes at 3 PM, RRB previous year papers with solutions, topic-wise MCQs and full-length mock tests with 1/3 negative marking. Call or WhatsApp us at +91-8078686728 for free counselling.
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- AIIMS CRE Lab Technician Question Paper 2026 PDF
- DMLT 2nd Year Syllabus 2026
Conclusion
The RRB Lab Assistant Grade II paper of 11 March 2026 was easy to moderate, with Microbiology, Clinical Biochemistry and Blood Banking deciding the score. In the previous recruitment, the official UR cut off went up to 69.05 (RRB Mumbai – Central Railway), so target 70+ normalised marks. Download the question paper PDF with answer key and start preparing for the upcoming RRB CEN 05/2026 recruitment — the same lab concepts are repeated every year.
