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B.Sc. MLT 2nd Year (NCAHP name: Bachelor of Medical Laboratory Science – B.MLS) covers Semester 3 and 4. Semester 3: Bacteriology, Intermediary Metabolism & Endocrinology, Clinical Haematology, Basics of Pharmacology. Semester 4: Virology & Immunology, Genetics & Molecular Biology, Clinical Pathology, Medical Laboratory Management & Quality Control — each main subject with a practical. Each semester has 20 credits and 700 marks.

Whether your university calls it BMLT, B.Sc. MLT, B.Sc. Medical Lab Technology or BMLS, the national standard is the NCAHP Competency Based Curriculum for Medical Laboratory Sciences (released 15 October 2025, implementation from 2026–27). This post gives the complete 2nd year (Semester 3 & 4) syllabus with credits, marks and unit-wise topics.

B.Sc. MLT 2nd Year at a Glance

Particulars Details
Course Bachelor of Medical Laboratory Science (B.MLS) – popularly B.Sc. MLT / BMLT
2nd Year Semesters Semester 3 and Semester 4
Credits 20 per semester (40 in 2nd year)
Marks 700 per semester (each paper 30 internal + 70 end-semester)
Attendance 80% theory and 80% practical
Promotion to 3rd Year Cumulative minimum 80% of credits of Semester 1 to 4
Regulator National Commission for Allied and Healthcare Professions (NCAHP)

B.Sc. MLT 2nd Year – Semester 3 Syllabus

Subject Credits Marks (Int + Ext)
Bacteriology 4 30 + 70 = 100
Intermediary Metabolism and Endocrinology 4 30 + 70 = 100
Clinical Haematology 4 30 + 70 = 100
Basics of Pharmacology 2 30 + 70 = 100
Bacteriology Practical 2 30 + 70 = 100
Intermediary Metabolism and Endocrinology Practical 2 30 + 70 = 100
Clinical Haematology Practical 2 30 + 70 = 100
Total 20 700

Semester 3 – Unit-wise Topics

Subject Main Topics
Bacteriology Gram-positive cocci, Gram-negative cocci, spore-forming bacteria, mycobacteria, anaerobes, Enterobacteriaceae, Vibrio, Pseudomonas & Burkholderia, Mycoplasma, Actinomycetes & Nocardia, types of infections & diagnosis, nosocomial (hospital) infections
Intermediary Metabolism and Endocrinology Metabolism of carbohydrates, lipids and proteins, integration of metabolism, purine & pyrimidine metabolism, hormones
Clinical Haematology Anaemias, leukaemia, haemostasis disorders, cytogenetics (karyotyping, FISH), basics of flow cytometry with CD markers
Basics of Pharmacology General pharmacology, antimicrobial chemotherapy, drugs for hormonal & metabolic disorders, drugs acting on blood and blood-forming organs

B.Sc. MLT 2nd Year – Semester 4 Syllabus

Subject Credits Marks (Int + Ext)
Virology and Immunology 4 30 + 70 = 100
Genetics and Molecular Biology 4 30 + 70 = 100
Clinical Pathology 4 30 + 70 = 100
Medical Laboratory Management and Quality Control 2 30 + 70 = 100
Virology and Immunology Practical 2 30 + 70 = 100
Genetics and Molecular Biology Practical 2 30 + 70 = 100
Clinical Pathology Practical 2 30 + 70 = 100
Total 20 700

Semester 4 – Unit-wise Topics

Subject Main Topics
Virology and Immunology Introduction to virology, cultivation & detection of viruses, lab diagnosis of viral infections, medically important viruses, prevention & precautions in virology; immune system, antigen & antibody, complement system, autoimmune diseases, immunisation/vaccination
Genetics and Molecular Biology Introduction to genetics, chromosomal basis of inheritance, cell division, molecular basis of inheritance (DNA structure), gene expression & regulation, molecular diagnostics
Clinical Pathology Urine analysis, faecal (stool) analysis, semen analysis, sputum analysis, body fluids, recent advances in clinical pathology
Medical Laboratory Management and Quality Control Total quality management of clinical laboratory, accreditation & certification (NABL), automation in laboratory workflow, laboratory information system, sustainability of clinical laboratories

Old Annual Pattern – B.Sc. MLT 2nd Year Papers (Examples)

University 2nd Year Papers (Annual System)
AIIMS Bhubaneswar – B.Sc. (Hons.) MLT Transfusion Medicine; Pathology-II (Special Haematology); Microbiology-II (Bacteriology & Parasitology); Biochemistry-II (Clinical Biochemistry)
ABVMU Lucknow (UP) – BMLT Blood Bank Procedure & Haemoglobinopathies; Endocrinology, Tumour & Cancer Markers; Clinical Biochemistry; Immunology, Serology, Parasitology

Course names in states: Rajasthan (RUHS) – B.Sc. (Medical Lab Technology); UP (ABVMU) – BMLT / BMLS; Bihar (AKU) – BMLT; AIIMS – B.Sc. (Hons.) MLT; NCAHP – B.MLS. Confirm the exact subjects with your university.

Exam & Promotion Rules (NCAHP)

Rule Details
Marks per Paper 100 = 30 continuous assessment + 70 end-semester exam
Grading A+ (10), A (9), B (8), C (7), D (6), E (5), F (0)
Attendance 80% in theory and 80% in practical
2nd → 3rd Year Promotion Cumulative minimum 80% of credits at the end of 2nd year (Semester 1 to 4)

How to Score Well in B.Sc. MLT 2nd Year

Tip What to Do
Bacteriology charts Make a chart of organisms – Gram stain, culture media, key tests
Haematology slides Practise peripheral smear and blood cell identification daily
Clinical pathology Learn normal values of urine, stool, semen and body fluids
Quality control Understand Levey-Jennings chart, Westgard rules and NABL basics – asked in govt exams too

Conclusion

B.Sc. MLT 2nd year moves from basics to core lab subjects — bacteriology, clinical haematology, virology & immunology, molecular biology and clinical pathology — in Semester 3 and 4 as per the NCAHP B.MLS curriculum.

Other years: B.Sc. MLT 1st Year Syllabus · B.Sc. MLT 3rd Year Syllabus · More Lab Technician Guides

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Source: NCAHP Competency Based Curriculum for Medical Laboratory Sciences (B.MLS), released 15 October 2025 — ncahp.gov.in.